anti human mouse cd11b apc cy7  (Cytek Biosciences)

Journal: Cell reports

Article Title: Transcriptional programming mediated by the histone demethylase KDM5C regulates dendritic cell population heterogeneity and function

doi: 10.1016/j.celrep.2024.114506

Figure Lengend Snippet: (A) Representative plots and frequencies of splenic XCR1 + cDC1s, XCR1 − CD172a − merocytic DCs (mero), CLEC12A − cDC2A, and CLEC12A + cDC2B of total cDCs (Lin − MHCII + CD11c + CD26 + ) or of total cDC2s (CD172a + ) in female control (top) and Kdm5c ΔItgax (bottom) mice. (B) Representative plots, proportions, and counts of splenic Ly6C + pDCs and Ly6C − pDCs from female control and Kdm5c ΔItgax mice (parent gate: Lin(B220) + SiglecH + CD11c int CD11b − ). (C) BM chimeras were generated in CD45.1 + host mice by reconstituting their BM with a 1:1 mixture of CD45.1 + control and CD45.2 + Kdm5c ΔItgax BM or CD45.2 + Kdm5c ΔItgax BM alone. Proportions of splenic DC subsets are derived from CD45.1 + control and CD45.2 + Kdm5c ΔItgax cells 7 weeks post injection. (D) Proportions of splenic cDCs of mice administered α-IgG2b or α-PDCA1 to deplete pDCs. (E) Frequencies of splenic cDCs from control and Ifnar1 −/− mice. Each symbol represents an individual mouse. Data were pooled from 2–3 experiments of 9–11 mice per group. (A, B, D, and E) Statistical significance was determined by unpaired t test or (C) one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. All error bars represent mean and SEM. See also .

Article Snippet: Anti-mouse CD11b APC/Cy7 (clone M1/70) , eBioscience , 47-0112-82; RRID:AB_1603193.

Techniques: Control, Generated, Derivative Assay, Injection

Journal: Cell reports

Article Title: Transcriptional programming mediated by the histone demethylase KDM5C regulates dendritic cell population heterogeneity and function

doi: 10.1016/j.celrep.2024.114506

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse CD11b APC/Cy7 (clone M1/70) , eBioscience , 47-0112-82; RRID:AB_1603193.

Techniques: Control, Blocking Assay, Virus, Recombinant, Protease Inhibitor, Lysis, Enzyme-linked Immunosorbent Assay, Staining, Cell Isolation, DNA Library Preparation, Purification, RNA HS Assay, Mutagenesis, Software

Journal: bioRxiv

Article Title: Unbiased metastatic niche-labeling identifies estrogen receptor-positive macrophages as a barrier of T cell infiltration during bone colonization

doi: 10.1101/2024.05.07.593016

Figure Lengend Snippet: (A) Cell types, tissue origin, and biotin groups in SAMENT single-cell dataset. (B) GSVA analysis comparing biotin-positive NK cells to biotin-negative NK cells highlights potential regulations exerted by NK cells on macrophages. Biotin-positive NK cells appear to modulate the tumor microenvironment by enhancing chemotaxis and inhibiting both aging and apoptosis in macrophages. This suggests that upon encountering tumors, reprogrammed NK cells indirectly contribute to chronic inflammation and promote the survival of tumor-associated macrophages. (C) Top enriched pathways in biotin-positive immature B cells in bones compared with other tissues. (D) Top enriched pathways in monocyte and neutrophil precursors in lungs compared with other tissues. (E) Top enriched pathways in biotin-positive granulocyte-macrophage progenitors (GMPs) in bone compared with biotin-negative populations. (F) Validation of published tissue-specific macrophage signatures was conducted in the SAMENT dataset. Reported Microglia signatures include Tmem119 + , Trem2 + , Fcrls + , Slc2a5 + , P2ry12 + , and Cx3cr1 + . Alveolars signatures consist of Cd163 − , Cd11b − , Cd206 int , Spp1 + , SiglecF + and Cd11c + ; Osteoclasts signatures are characterized by Arg1 + , Spp1 + , Mmp9 + , and vacuolar [H+]-ATPase + ; Kupffers cell signatures include CD11b low , F4/80 high , and Clec4F + ; Monocyte-derived macrophage signatures , encompass CD11b + , F4/80 int , Ly6C + and CSF1R + . In our dataset, Mφ_c10 corresponds to Microglia, Mφ_c7 corresponds Alveolar macrophages, and Mφ_c5 corresponds to osteoclasts. Monocyte-derived macrophages are distributed across all macrophage subclusters, while we did not identify specific clusters for Kupffer cells. These findings support the observations depicted in .

Article Snippet: The antibodies used in this study included: Immune cell panel, CD45-VF450 (Cytek® Biosciences,75–0451), CD11b-APC/Cy7 (Cytek® Biosciences, 25–0112), Ly6G-Percp/Cy5.5 (Cytek® Biosciences, 65–1276), Ly6C-PE/Cy7 (BioLegend,128018), F4/80-BV510 (BioLegend,123135), B220-APC/Cy7 (Cytek® Biosciences, 25-0452), CD3e-Percp/Cy5.5 (Cytek® Biosciences,65-0031), CD4-PE/Cy7 (Cytek® Biosciences,60-0041), CD8a-BV711 (BD Biosciences,752634), PD-1-BV605 (BioLegend,135219), Biotin-APC (Miltenyi Biotec,130-113-288) or Biotin-PE(Miltenyi Biotec,130-113-291); Stromal cell panel, CD45-BV605 (BioLegend,103140), Ter119-BV605 (BioLegend,116239), CD31-APC/Cy7 (BioLegend,102440), Sca-1-Percp/Cy5.5 (eBioscience,45–5981-82), CD51-BV421 (BD Biosciences, 740062), CD140α-PE/Cy7 (BioLegend, 135912), Biotin-APC (Miltenyi Biotec,130-113-288) or Biotin-PE(Miltenyi Biotec,130-113-291) Flow cytometry analysis was conducted using a BD LSRFortessa flow cytometer and data were further analyzed with FlowJo software.

Techniques: Chemotaxis Assay, Biomarker Discovery, Derivative Assay

Journal: iScience

Article Title: Engraftment of adult hematopoietic stem and progenitor cells in a novel model of humanized mice

doi: 10.1016/j.isci.2024.109238

Figure Lengend Snippet:

Article Snippet: Mouse CD11b-APC/Cy7 (M1/70) , TONBO , 25-0112-U100; RRID: AB_3094465.

Techniques: Recombinant, Staining, Red Blood Cell Lysis, Enzyme-linked Immunosorbent Assay, Software